HeLa Ohio
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Description
Description: Derivative of parent HeLa line (ECACC catalogue no. 93021013).
Also Known As:
Species: Human
Tissue: Cervix
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr D Tyrrell, MRC Common Cold Unit, Salisbury
Originator: Yes
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 84121901
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