Hepa 1-6
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Description
Description: Derived from BW7756 tumour in a C57L mouse. Secretes several liver products including albumin, alpha-fetoprotein, alpha 1-antitrypin and amylase.
Also Known As:
Species: Mouse
Tissue: liver
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Darlington GJ. 1987 Liver cell lines. Methods Enzymol.;151:19-38. PMID: 3431441.
Hyperlink to ECACC Cell Line Data Sheet: 92110305
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