HKT-1097
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Description
Description: The cell line HKT-1097 has been established from a 14 month old male Syrian Hamster (Mesocricetus auratus) kidney tumour which has been induced by diethylstilbestrol exposure. HKT-1097 cells express epidermal growth factor (EGF) receptor, Erb-B2 and Erb-B3 as well as vimentin, desmin, glial fibrillary acidic protein (GFAP), TGF alpha, PGP 9.5 and S100 protein and neuron-specific enolase. Karyotype analysis revealed a modal number of 44 chromosomes (41% of cells) and polyploidy in approximately 2% of cells. The cells have a doubling time of about 36 hours.
Also Known As:
Species: Hamster
Tissue: Kidney
Growth Properties: Adherent
Morphology: Fibroblast-like
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 1 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Form a multipolar, loosely packed monolayer.
Release Conditions: No
Depositor: Dr G Laurent, Laboratory of Histology and Experimental Cytology, Faculty of Medicine and Pharmacy, University of Mons-Hainaut, Belgium
Originator: Yes
References: In Vitro Cell Dev Biol 1999; 35:339, In Vitro Cell Dev Biol – Animal 36: 640-649, 2000
Hyperlink to ECACC Cell Line Data Sheet: 98061003
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