HL60 Ast.4
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Description
Description: Variant of the promyelocytic cell line HL60 isolated after treating cells with alpha-particle radiation and then cloned in medium containing 1.25% DMSO. Treatment with 50nM TPA results in only very limited differentiation towards basophilic morphology (Giemsa stain). Differentiation towards monocyte lineage has not been observed.
Also Known As:
Species: Human
Tissue: Blood
Growth Properties: Suspension
Morphology: Promyeloid, blast/promyelocyte
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).
Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C. Do not allow the culture medium to become acidic.
Release Conditions: No
Depositor: Dr C M Bunce, Immunology Dept, University of Birmingham Medical School
Originator: Yes
References: Exp Haematol 1983;11:828
Hyperlink to ECACC Cell Line Data Sheet: 88120802
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