HL60 M2
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Description
Description: HL60 induced to mature into neutrophils with 1.75% DMSO after treating with SV40. Fails to express SV40 specific T Ag suggesting that HL60 M2 represents expansion of sub-clones of HL60 with inherent restricted capacity for neutrophil differentiation.
Also Known As:
Species: Human
Tissue: Blood
Growth Properties: Suspension
Morphology:
Growth Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).
Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml. Do not allow the culture medium to become acidic or the cells will differentiate; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr G Brown/Dr C M Bunce, Immunology Department The Medical School, University of Birmingham
Originator: Yes
References: Exp Haematol 1983;11:828; Leukemia Res 1982;6:491
Hyperlink to ECACC Cell Line Data Sheet: 88120803
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