HRA-16
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Description
Description: Established from the rectum of a 56 year-old male patient. Epithelial-like moderately differentiated adenocarcinoma. Imperial College Innovations has stated that this cell line is not to be supplied to commercial organisations.
Also Known As:
Species: Human
Tissue: Rectum
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:10 using 0.25% trypsin/EDTA; 7.5% CO2; 37°C. Suggested seeding density 2-4×10,000 cells/cm².
Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.
Depositor: Dr S Kirkland, Department of Histopathology, Imperial College London, Hammersmith Campus, DuCane Road, London, W12 0NN
Originator: Yes
References: S. C. Kirkland and I. G. Bailey. (1986) Establishment and characterisation of six human colorectal adenocarcinoma cell lines. Br J Cancer,53,6, 779-85.
Hyperlink to ECACC Cell Line Data Sheet: 06061905
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