HT 1080

CODE: 85111505
Human fibrosarcoma

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Description

Description: Established from a fibrosarcoma arising adjacent to the acetabulum of a 35 year old caucasian male. The cells can give rise to tumours in immunosupressed NIH Swiss mice. Susceptible to infection by RNA tumour viruses including RD114 feline endogenous virus and FeLV.

Also Known As:

Species: Human

Tissue: Fibrosarcoma

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4×10000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: Cancer 1974;33:1027

Hyperlink to ECACC Cell Line Data Sheet: 85111505

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