HT29
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Description
Description: Isolated from a primary tumour in a 44 year old Caucasian female. Forms a well-differentiated adenocarcinoma consistent with colony primary, grade I. Tumours also form in steroid treated hamsters. Has the following HLA profile A1,3; B12,17; Cw5.
Also Known As:
Species: Human
Tissue: Colon
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: McCoy’s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3×10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Human Tumor Cells In Vitro, 1975:115. Plenum Press, NY
Hyperlink to ECACC Cell Line Data Sheet: 91072201
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