HTC (BUdR)
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Description
Description: Clone of the original HTC hepatoma (ECACC catalogue no. 93120108) rendered resistant to 10ug/ml BUdR, which must be grown in HAT. The cells are used in enzyme induction studies.
Also Known As:
Species: Rat
Tissue: Liver
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1%HAT + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1966;56:296
Hyperlink to ECACC Cell Line Data Sheet: 85061110
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