HTK
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Description
Description: The cells are rendered TK- by selection with 50µg/ml BUdR. HTK- has the same STR profile as 143B (ECACC Catalogue number 91112502)”, both are TK-; it is likely that they are the same cell line by different names.
Also Known As:
Species: Human
Tissue: Bone
Growth Properties: Adherent
Morphology:
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 25mg/l Bromo-deoxy Uridine (BUdR) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr A Collins, AFRC Institute for Research on Animal Diseases, Compton
Originator: Yes
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 88022409
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