Description: Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.

Also Known As:

Species: Human

Tissue: Liver

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: DMEM + 10% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37 C. Media change twice a week

Release Conditions: Yes all organisations are required to complete a Cell Line Release Authorisation form

Depositor: Prof John Monjardino, Department of Medicine, Imperial College School of Medicine (St. Mary's) Norfolk Place, London W2 1NY

Originator: Yes

References: Cheng D, Yang A, Thomas H, Monjardino J.1993 Characterization of stable hepatitis delta expressing hepatoma cell lines: effect of HDAg on cell growth. Prog Clin Biol Res. 382:149-53. PMID: 8502673.

Hyperlink to ECACC Cell Line Data Sheet: 01042712