HuP-T4
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Description
Description: Derived from an ascites sample taken from a 60 year old Japanese male with a well differentiated pancreatic adenocarcinoma. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Also Known As:
Species: Human
Tissue: pancreas
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor: Dr S Saito, Toyama Medical and Pharmaceutical University, Toyama
Originator: Yes
References: Int J Pancreatol 1993;13(1):31
Hyperlink to ECACC Cell Line Data Sheet: 93121056
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