IA-Xs SBR

CODE: 88090801
Holtzmann rat small bowel adenocarcinoma

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Description

Description: Derived from X-irradiation induced small bowel adenocarcinoma in adult male Holtzmann rat. Sensitive to lysis by peripheral blood lymphoid cells taken from F344 rats with colon and pancreatic cancers induced by 1, 2 Dimethylhydrazine or 7, 12 DMBT.

Also Known As:

Species: Rat

Tissue: Bowel

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: Ham’s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 4×10, 000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor:

Originator: No

References: J Nat Cancer Inst 1977;59:1315

Hyperlink to ECACC Cell Line Data Sheet: 88090801

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