ICR-2A
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Description
Description: Previously designated RPH68.2A. Derived from androgenetic haploid embryos of the grass or leopard frog, (Rana pipiens). The cells retain their haploid chromosome complement for over 200 generations. Used in genetic studies.
Also Known As:
Species: Frog
Tissue: Embryo
Growth Properties: Adherent
Morphology: Fibroblast-like
Growth Medium: L15 (50%) + Distilled Water (40%) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5×10,000 cells/cm² using 0.25% trypsin; 25°C. Do not use CO2. Grow cells in dark (i.e wrapped in foil). Cells do not reach 100% confluency, only approximately 70-80%. Freeze in glycerol.
Release Conditions: No
Depositor:
Originator: No
References: Proc Natl Acad Sci, USA 1970;65:337
Hyperlink to ECACC Cell Line Data Sheet: 89072615
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