IIC9 (CH0)

CODE: 00030814
Chinese hamster embryo fibroblast

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Description

Description: This cell line is a selected subclone (IIC9) of the CHEF 18 Chinese Hamster embryo cell line

Also Known As:

Species: Hamster

Tissue: Embryo

Growth Properties: Adherent

Morphology: Fibroblast

Growth Medium: MEM/F12 1:1 + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:10 ie. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin /EDTA; 37°C; 5% CO2.

Release Conditions: No

Depositor: Dr D M Raben, Department of Medicine, John Hopkins University School of Medicine, Baltimoe, Maryland. 21205

Originator:

References: Journal of Biological Chemistry 1993 268 9215-9222. PubMed=7088192; DOI=10.1038/298476a0; Low D.A., Scott R.W., Baker J.B., Cunningham D.D.; "Cells regulate their mitogenic response to thrombin through release of protease nexin."; Nature 298:476-478(1982).

Hyperlink to ECACC Cell Line Data Sheet: 00030814

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