IMR-90
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Description
Description: Derived from lung tissue of a 16 week old female Caucasian foetus. The cells have a virus susceptibility similar to WI-38 (ECACC catalogue no. 90020107) and MRC-5 cells (ECACC catalogue no. 84101801). The cells have a finite lifespan. The current batch of these cells have been shown to be capable of at least 10 further population doublings before senescence.
Also Known As:
Species: Human
Tissue: Lung, foetal
Growth Properties: Adherent
Morphology: Fibroblast
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Science 1977;196:60
Hyperlink to ECACC Cell Line Data Sheet: 85020204
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