J774.2
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Description
Description: Recloned from J774.1 original ascites and solid tumour. Produces IL-1.
Also Known As:
Species: Mouse
Tissue: blood
Growth Properties: Semi-adherent
Morphology:
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Maintain cultures between 3-9×100,000 cells/ml; 5% CO2; 37°C. Cells are semi-adherent i.e. some cells grow in suspension , some loosely attach to the surface and others flattened out and attach to the flask. They should be passaged by scraping. Cells should not be allowed to overgrow and become confluent as this can lead to a loss of the flattened adherent cell characteristic.
Release Conditions: No
Depositor:
Originator: No
References: J Immunol 1975;114:898; Cancer Research 1977;37:546
Hyperlink to ECACC Cell Line Data Sheet: 85011428
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