J774A.1
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: Derived from a tumour in a female BALB/c mouse. Cell line produces IL-5 and lysozyme.
Also Known As:
Species: Mouse
Tissue: Blood
Growth Properties: Semi-adherent
Morphology:
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Cells are subcultured by harvesting attached and suspension cells. Remove majority of medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Seed into new flask at 1:3 to 1:4 split ratio i.e. seeding at 1-3 x 10000 cells/cm2 Incubate at 37°C; 5% CO2. Please Note :- On receipt growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet. This should then be re-suspended in fresh media and a cell count performed. The cells should then be reseeded at 2-4 x 10E4/cm and should begin to show signs of re-attachment within 48 hours.
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1977;37:546; J Immunol 1977;119:950; J Immunol 1975;114:898
Hyperlink to ECACC Cell Line Data Sheet: 91051511
Ask a Question
Have a question about this product? Our technical team is here to help.
