JEG3
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Description
Description: One of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. Cell line releases chorionic gonadotrophin and somatomammotrophin and progesterone. It is also able to transform steroid precursors to oestrone and oestradiol. JEG3 has the same DNA profile as BeWo (ECACC catalogue number 86082803); it was established by serial cloning of BeWo (see Pattillo & Gey 1968 PMID: 4299001).
Also Known As:
Species: Human
Tissue: Placenta
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3×10,000 cells/cm² using 0.25% trypsin, 5% CO2; 37°C. Vacuolisation will occur at confluency.
Release Conditions: No
Depositor:
Originator: No
References: Kohler PO, Bridson WE 1971 Isolation of hormone-producing clonal lines of human choriocarcinoma. J Clin Endocrinol Metab.; 32(5):683-7.PMID: 5103722.
Hyperlink to ECACC Cell Line Data Sheet: 92120308
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