Description: Cell morphology is different from the parent clone (A31) and there is a loss of sensitivity to contact inhibition of cell division. The cells are highly tumorigenic. Superinfection with MuLV results in rescue of MSV.

Also Known As:

Species: Mouse

Tissue: Embryo

Growth Properties: Adherent

Morphology: Fibroblast

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10000 cells/cm² using 0.1% trypsin for 3-5 minutes; 5% CO2; 37°C

Release Conditions: No

Depositor:

Originator:

References: J Gen Virol 1971;13:245

Hyperlink to ECACC Cell Line Data Sheet: 89092101