Description: A human B cell lymphoma established from a pleural effusion of a 23-year-old woman with mediastinal lymphoblastic B-cell non-Hodgkin's lymphoma. This cell line does not express BCL2.

Also Known As:

Species: Human

Tissue: Blood (peripheral)

Growth Properties: Aggregates in suspension

Morphology: Lymphoblastoid

Growth Medium: RPMI 1640 + 2mM Glutamine + 10 – 20% Foetal Bovine Serum (FBS)

Subculturing Procedure: If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 – 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 – 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 – 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%.Maintain cultures between 3 – 9 x100,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 60-100 hours. Spherical cells growing in clusters in suspension.

Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.

Depositor: Dr Abraham Karpas, Department of Haematology, University of Cambridge, MRC Centre, Hills Road, Cambridge, UK

Originator: Yes

References: Nacheva et al., (1994) B-cell non-Hodgkin's lymphoma cell line (Karpas 1106) with complex translocation involving 18q21.3 but lacking BCL2 rearrangement and expression. Blood 84, 3422-8.

Hyperlink to ECACC Cell Line Data Sheet: 06072607