KARPAS 45
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Description
Description: A human T cell leukaemia cell line established from the bone marrow of a 2-year-old boy diagnosed with acute lymphoblastic leukaemia (ALL) prior to therapy in 1972; cells were reported to carry the MLL-AFX and AFX-MLL fusion genes, the original diagnosis was T-ALL.
Also Known As:
Species: Human
Tissue: Bone marrow
Growth Properties: Suspension
Morphology: Spherical cells
Growth Medium: RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS). For slower growth use 10% or 5% FBS.
Subculturing Procedure: Maintain cultures between 0.5 – 2 x1000,000 cells/ml; 5% CO2; 37°C. Doubling time 50-100 hours. Split saturated culture 1:2 to 1:3 every 2-3 days. Spherical cells growing in clusters in suspension.
Release Conditions: Yes – commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.
Depositor: Dr Abraham Karpas, Department of Haematology, University of Cambridge, MRC Centre, Hills Road, Cambridge, UK
Originator: Yes
References: Karpas et al., (1977) Leukemia Research 1:35-49, Smith et al.,” (1973) Lancet: 74-77
Hyperlink to ECACC Cell Line Data Sheet: 06072602
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