KHOS-240S
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Description
Description: A revertant line of the KHOS/NP cell line (ECACC catalogue no. 84102903). Established after overnight incubation at 40.5°C and then sub-cloned at 36°C. The line has similar properties to the parent HOS cell line. Cells are non-tumourigenic in nude mice and no longer possess a rescuable MSV genome.
Also Known As:
Species: Human
Tissue: Bone
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4×10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: J Gen Virol 1979;43:447; Science 1979;205:691
Hyperlink to ECACC Cell Line Data Sheet: 86112810
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