L5178Y tk+/- 3.7.2c (IVGT)
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Description
Description: The L5178Y tk+/- 3.7.2c (IVGT) mouse lymphoma cell line was derived from a thymic tumour induced in a DBA/2 mouse by methylcholanthrene treatment. The cell line is tumourigenic in DBA/2 mice. The cell line can be used in genotoxicity tests including gene mutation assays at the tk and other loci and micronucleus induction.
Also Known As:
Species: Mouse DBA/2
Tissue: Thymus
Growth Properties: Suspension
Morphology: Small rounded cells growing in suspension
Growth Medium: Fischer's medium + 2mM Glutamine + 1% Sodium pyruvate + 10% heat-inactivated Horse Serum (HS)
Subculturing Procedure: Maintain cultures in logarithmic growth phase between 3 x 104 and 1 x 1,0, 000 cells/ml.
Release Conditions: No
Depositor:
Originator: No
References: Sawyer JR, Binz RL, Wang J, Moore MM. 2006 Multicolor spectral karyotyping of the L5178Y Tk+/- -3.7.2C mouse lymphoma cell line. Environ Mol Mutagen.47(2):127-31. PMID: 16247762
E. Lorge, M.M. Moore, J. Clements, M. O’Donovan, M.D. Fellows, M. Honma, A. Kohara, S. Galloway, M.J. Armstrong, V. Thybaud, B. Gollapudi, M.J. Aardema, J.Y. Tanir. 2016 Standardized cell sources and recommendations for good cell culture practices in genotoxicity testing. Mutation Research. 809: 1-15.
Hyperlink to ECACC Cell Line Data Sheet: 12080201
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