L8
[Create a profile and sign in to view pricing and add to cart]
- Description
- Ask a Question
Description
Description: The myogenic cell line L8 was isolated by D Yaffe in 1969 by selective serial passage of myoblasts from primary rat skeletal muscle cell cultures (new born, non-in-bred Wistar rats). Subsequently they were recloned by H Blau. L8 cells differentiate into cross-striated myotubes by fusion and express several muscle-specific proteins. The myoblast population will be depleted if cells are allowed to become confluent before sub-culturing. Therefore, sub-cultivation before confluency and periodical re-cloning is necessary. The preparation of a frozen stock at early passage is recommended.
Also Known As:
Species: Rat
Tissue: Skeletal muscle
Growth Properties: Adherent
Morphology: Myoblast
Growth Medium: DMEM : Medium 199 (4:1) + 2mM Glutamine + 1% CEE (Chick Embryo extract (Seralab)) + 10% Horse Serum (HS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5×1,000 cells to 1×10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C; sub-cultivate before confluency and reclone periodically.
Release Conditions: No
Depositor:
Originator: No
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 95102434
Ask a Question
Have a question about this product? Our technical team is here to help.
