LB198
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Description
Description: LB198 has been derived from mononuclear cells from peripheral blood of a 45 year old female. The cells have been transformed with EBV. The patient has been shown to be heterozygote for trichothiodystrophy (TTD) and is the parent of LB199 (ECACC catalogue no.98062321) TTD is a rare genetic disorder with deficiency in excision repair of UV damage. Fibroblasts from the same individual have been designated TTD HM 1BR.
Also Known As:
Species: Human
Tissue: Blood
Growth Properties: Suspension
Morphology: Lymphoblastoid
Growth Medium: RPMI 1640 + 4mM Glutamine + 200 µg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form floating aggregates.
Release Conditions: No
Depositor: Dr. J. Cole, MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, East Sussex, BN1 9RR
Originator: Yes
References:
Hyperlink to ECACC Cell Line Data Sheet: 98062320
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