LB443
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Description
Description: Established by EBV transformation of mononuclear cell from a peripheral blood sample from a 4 year old male patient. The patient was diagnosed with trichothiodystrophy (TTD)”, a syndrome connected to deficiency in excision repair of ultraviolet radiation induced DNA damage. Studies with fibroblasts from the same patient, known as TTD4BR, have been published.
Also Known As:
Species: Human
Tissue: Blood
Growth Properties: Suspension
Morphology: Lymphoblastoid
Growth Medium: RPMI 1640 + 4mM Glutamine + 200 µg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C
Release Conditions: No
Depositor: Dr. J. Cole, MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, East Sussex, BN1 9RR
Originator: Yes
References: PNAS USA 1997; 94:6837
Hyperlink to ECACC Cell Line Data Sheet: 98062322
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