LB81
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Description
Description: Established by EBV transformation of mononuclear cells from a peripheral blood sample from a 37 year old female patient. The patient was shown to be heterozygote for Xeroderma pigmentosum complementation group G, a rare, autosomal recessive disease, and is the mother of two children with XP (cell line LB83, ECACC catalogue no. 98062328 and LB82).
Also Known As:
Species: Human
Tissue: Unknown
Growth Properties: Suspension
Morphology: Lymphoblastoid
Growth Medium: RPMI 1640 + 4mM Glutamine + 200 µg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).
Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form free floating aggregates.
Release Conditions: No
Depositor: Dr. J. Cole, MRC Cell Mutation Unit, University of Sussex, Falmer, Brighton, East Sussex, BN1 9RR
Originator: Yes
References: Mutation Res 1992; 273: 171
Hyperlink to ECACC Cell Line Data Sheet: 98062327
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