Description: LIM 1899 is a columnar cell adenocarcinoma of the colon.

Description Key Words: Columnar cell adenocarcinoma colon

Also Known As: No other names

Organism: Human (Homo sapiens)

Strain: N/A

Tissue: Colon

Growth Properties: Adherent

Morphology: Epithelial. Cells are small rounded and grow in clusters.

Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% Foetal Calf Serum (FCS) + Insulin 0.6μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10μM.

Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.

Subculturing Procedure:

Medium Renewal: 3 times each week.

Subcultivation Ratio: 1:8 Seeding density 1×10⁴ cells/cm². Split sub confluent flasks (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.

Culture Conditions: Cells maintained at 37°C and 5% CO₂.

Cryoprotectant Medium: 10% DMSO + 90% FCS

Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.

Additional Information: Sporadic CRC. Mutated beta-catenin and K-Ras. A33 +ve.

Depositor: Professor Tony Burgess – Ludwig Institute for Cancer Research Ltd, Melbourne, Australia

References:

Zhang H. et al. Selective inhibition of proliferation in colorectal carcinoma cell lines expressing mutant APC or activated B-Raf. Int.J.Cancer 2009 July 15; 125(2):297-307. PubMed 19378335.

Andrew S.Met al. Tumor localization by combinations of monoclonal antibodies in a new human colon carcinoma cell line. Cancer Res 1990 September 1; 50(17):5225-5230. PubMed 2386931.

Cellosaurus: CVCL_4435