Description: Adherent cell line derived from adenocarcinoma of the caecum of a male patient.

Description Key Words: Human adenocarcinoma caecum male 

Also Known As: No other names

Organism: Human (Homo sapiens)

Strain: N/A

Tissue: Colon

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% Foetal Calf Serum (FCS) + Insulin 0.6μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10μM.

Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.

Subculturing Procedure:

Medium Renewal: 3 times each week.

Subcultivation Ratio: 1:4 – 1:6 Seeding density 1.6 x10⁴ cells/cm². Split sub-confluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes. Cells may take 2-3 days to seed after thawing or trypsinisation. 

Culture Conditions: Cells maintained at 37°C and 5% CO₂.

Cryoprotectant Medium: 10% DMSO + 90% FCS

Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.

Additional Information: Cells are adherent, spindly, grow as xenografts.

• Mutations:
  • Heterozygous APC mutation (stop at aa 2198)
  • B-Raf mutation (V600E)
• MSI
• A33 negative

Depositor: Professor Tony Burgess – Ludwig Institute for Cancer Research Ltd, Melbourne, Australia

Reference:

R.H. Whitehead , H.H. Zhang, I.P. Hayward. Retention of tissue specific phenotype in a panel of colon carcinoma cell lines: Relationship to clinical correlates. Immunol Cell Biol. 1992; 70: 227-36. PubMed 1427983.

Cellosaurus: CVCL_4437