Description: Human merkel cell carcinoma cell line

Description Key Words: Human merkel cell carcinoma 

Also Known As: No other names

Organism: Human (Homo sapiens)

Tissue: Skin

Growth Properties: Adherent

Morphology: Large low contrast cells

Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM Hepes) + 15% Foetal Calf Serum (FCS)

Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.

Subculturing Procedure:

Medium Renewal:

Subcultivation Ratio: 1:8 Seeding density 10⁴ cells /cm². Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.

Culture Conditions: Cells incubated at 37°C with 5% CO2. 

Cryoprotectant Medium: 10% DMSO + 90% FCS

Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability. 

Depositor: Helen Leonard – Queensland Institute of Medical Research, Australia

References:

Original Reference:

Leonard, J.H., Dash, P., Holland,.P., Kearsley, J.H. and Bell, J.R.

Characterization of Merkel cell carcinoma adherent cell lines.

Int. J Cancer, 60: 100-107, 1995.

PubMed 7814141

Cellosaurus: CVCL_2584