Description: LIM2537 is an adherent cell line derived from a primary colonic carcinoma from a 73-year-old male patient.

Description Key Words: Human primary colon carcinoma from male patient 

Also Known As: No other names

Organism: Human (Homo sapiens)

Tissue: Carcinoma of the transverse colon

Growth Properties: Adherent and rounded the cells grow in tight islands

Morphology: Epithelial

Doubling Time: 30 hours

Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% Foetal Calf Serum (FCS) + Insulin 0.6μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10μM.

Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.

Subculturing Procedure:

Medium Renewal: 3 times each week.

Subcultivation Ratio: 1:8 Seeding density 1-2 x10⁴ cells/cm². Split sub-confluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes. 

Culture Conditions: Cells maintained at 37°C and 5% CO₂.

Cryoprotectant Medium: 10% DMSO + 90% FCS

Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.

Additional Information: Cells are adherent, spindly, grow as xenografts.

• Mutations:
  • Truncated APC mutation (heterozygous stop at aa 1472)
  • p53 mutation (G245D)
  • B-Raf mutation (V600E)
  • PI3K mutation (23 aa deletion in p85α)
• MSI
• A33 negative

Depositor: Professor Tony Burgess – Ludwig Institute for Cancer Research Ltd, Melbourne, Australia

Reference:

Philp AJ, Campbell IG, Leet C, Vincan E, Rockman SP, Whitehead RH, Thomas RJ, Phillips WA. The Phosphatidylinositol 3′-kinase p85α Gene Is an Oncogene in Human Ovarian and Colon Tumors Cancer Res. 2001 Oct 15;61(20):7426-9. PubMed 11606375.

Zhang H.H.”, Walker F., Kiflemariam S., Whitehead R.H., Williams D., Phillips W.A., Mikeska T., Dobrovic A., Burgess A.W. Selective inhibition of proliferation in colorectal carcinoma cell lines expressing mutant APC or activated B-Raf. Int.J.Cancer 2009 Jul 15; 125(2):297-307. PubMed 19378335

Cellosaurus: CVCL_4441