LL86 (LeSa)
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Description
Description: Derived from normal tissue of an 18 year old Caucasian male with osteogenic sarcoma.
Also Known As:
Species: Human
Tissue: lung
Growth Properties: Adherent
Morphology: Fibroblast-like
Growth Medium: Coon’s modified Ham’s F12 + Kaign’s modified Ham’s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3×10000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. A further 10 population doublings expected.
Release Conditions: No
Depositor:
Originator: No
References: None specified by depositor
Hyperlink to ECACC Cell Line Data Sheet: 90102541
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