Description: Derived from a metastasis at the left supraclavicular lymph node of a 50 year old patient with a confirmed diagnosis of metastatic prostate carcinoma. Growth and acid phosphatase production is affected by 5-alpha-dihydrotestosterone. They do not form a uniform monolayer and attach only lightly to the substrate. When shipped, cells detach from flask and can either be incubated 24-48 hours to allow attachment or be collected by centrifugation (150xg, 15 minutes) and reseeded.

Also Known As:

Species: Human

Tissue: Prostate

Growth Properties: Adherent

Morphology: Epithelial-like

Growth Medium: RPMI 1640 + 2mM Glutamine + 1.0 mM sodium pyruvate + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.i.e. seeding at 3-4 x 10,000 cells/cm². Cells grow slowly in clusters and can be disaggregated by repeated pipetting. After subculture cells may take up to 48 hours to reattach. Do not disturb during this period. Medium is rapidly acidified, therefore change twice per week.

Release Conditions: No

Depositor:

Originator: No

References: Cancer Genet Cytogenet 1984; 11:399; Cancer Res 1983; 43:1809; Cancer Res 1997; 57:3339; J Biol Chem 1996; 271:13228

Hyperlink to ECACC Cell Line Data Sheet: 89110211