Description: m17.ASC is a spontaneously immortalised multipotent mesenchymal cell line capable of acquiring adipocyte, chondrocyte, osteocyte and cardiomyocyte phenotypes when cultured with appropriate differentiation factors. m17.ASC provides a valuable tool for in vitro biomimetic studies; assessing growth technologies such as 3-D structures, biochemical cues (selected growth factors and hormones) and physical forces (electrical, mechanical and topographical). It can be used to screen compounds that induce or inhibit proliferation and/or differentiation in tissue specific phenotypes.

Also Known As:

Species: Mouse fvb strain

Tissue: Adipose

Growth Properties: Adherent

Morphology: Fibroblast-like

Growth Medium: Claycomb medium (Sigma 51800C) + 2mM glutamine + 10% FCS

Subculturing Procedure: Split sub-confluent cultures (90%) approximately 1:6 with media changes or subculture every 2-3 days seeding at 0.5-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Release Conditions: No

Depositor: Professor M Prat, Head of the Laboratory of Histology, Department of Medical Sciences, Universita del Piemonte Orientale, Italy

Originator: Yes

References: Zamperone A, Pietronave S, Merlin S, Colangelo D, Ranaldo G, Medico E, Di Scipio F, Berta GN, Follenzi A, Prat M. 2013 Isolation and characterization of a spontaneously immortalized multipotent mesenchymal cell line derived from mouse subcutaneous adipose tissue. Stem Cells Dev. 22(21):2873-84. PMID: 23777308

Hyperlink to ECACC Cell Line Data Sheet: 11112201