M3 Clone M-3
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Description
Description: Melanin producing cell line, derived from a Cloudman S 91 melanoma induced in a F1 (CxDBA) male mouse. The line is tumourigenic in mice and susceptible to a variety of viruses including herpes simplex, vaccinia, pseudorabies and vesicular stomatitis virus (Indiana virus).
Also Known As:
Species: Mouse
Tissue: skin
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: Ham’s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Release Conditions: No
Depositor:
Originator: No
References: Science 1966;154:1186, Proc. Natl. Acad. Sci. USA 94: 3262-3267, 1977
Hyperlink to ECACC Cell Line Data Sheet: 93120827
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