MCA-RH 7777
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Description
Description: Derived from the Morris hepatoma 7777 (obtained from a male Buffalo strain rat). The original tumour was induced with N-2 fluorenylphthalamic acid. Particularly interesting for oncogeny studies since the cells produce alpha-fetoprotein (AFP).
Also Known As:
Species: Rat
Tissue: liver
Growth Properties: Adherent
Morphology: Hepatocyte
Growth Medium: Swimm’s S-77 + 4mM Glutamine + 20% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split semi-confluent cultures 1:2 to 1:6 i.e. seeding at 1-3×10000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. NB: Cells are shed from the monolayer before confluency is achieved.
Release Conditions: No
Depositor:
Originator: No
References: Cancer Res 1974;44:103
Hyperlink to ECACC Cell Line Data Sheet: 90021504
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