Description: MCC26 is an adherent merkel cell carcinoma (MCC) cell line. It was established from the recurrence of a tumour in an 82 year old female. The lesion on the left lateral calf was confirmed by biopsy to be MCC.

Description Key Words: Human merkel cell carcinoma

Also Known As: No other names

Organism: Human (Homo sapiens)

Strain: N/A

Tissue: Skin

Growth Properties: Adherent

Morphology: Large low contrast adherent cells

Growth Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM Hepes) + 15% Foetal Calf Serum

Resuscitation: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.

Subculturing Procedure:

Medium Renewal: 2 times per week.

Subcultivation Ratio: 1:4 Seeding density 0.8 – 1.0 x10⁴cells/cm². Split subconfluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.

Culture Conditions: Incubate the culture at 37°C with 5% CO2.

Cryoprotectant Medium: 10% DMSO + 90% FCS

Handling Procedure for Frozen Cells: Upon receipt, frozen ampoules should be transferred directly to liquid nitrogen storage without delay, if not to be used immediately. Storage at -80°C may result in loss of viability.

Depositor: Helen Leonard – Queensland Institute of Medical Research, Australia

References:

Van Gele M, Leonard JH, Van Roy N, Van Limbergen H, Van Belle S, Cocquyt V, Salwen H, De Paepe A, Speleman F.Combined Karyotyping, CGH and M-FISH analysis allows detailed characterization of unidentified chromosomal rearrangements in merkel cell carcinoma. Int J Cancer, 10: 101(2):137-45. 2002 Sep. PubMed 12209990.

Leonard, J.H., Dash, P., Holland,.P., Kearsley, J.H. and Bell, J.R. Characterization of Merkel cell carcinoma adherent cell lines. Int. J Cancer, 60: 100-107, 1995. PubMed 7814141.

Cellosaurus: CVCL_2585