Description: The stable human mammary MCF7-derived reporter cell line called AREc32 contains copies of the rat Glutathione-S-transferase (GST) antioxidant response element (ARE) linked to a luciferase gene, such that the induction of the ARE results in luciferase activity.

ARE is a transcriptional regulatory element involved in the activation of genes coding for a number of antioxidant proteins and enzymes that work in concert to protect tissues from oxidative insults. This cell line can be used to investigate whether anticancer drugs can induce ARE-driven gene expression.

Also Known As:

Species: Human

Tissue: Breast

Growth Properties: Adherent

Morphology: Polygonal epithelium

Growth Medium: Dulbecco’s Moodifies Eagle Medium (DMEM) with 2.5 mM Glutamax supplemented with 10% foetal bovine serum. Do not culture beyond 15 passages after revival

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10⁴ cells/cm₂using 0.05% trypsin/EDTA solution. Cells are cultured at 37°C, in 95% air and 5% CO₂, and passaged every 3 to 4 days

Release Conditions: For research use only. Commercial organisations are required to fill in a ?Cell line release authorisation for research use in commercial organisations? form.

Depositor: Ximbio (formerly known as Cancer Research Technologies Limited)

Originator: Yes

References: Wang XJ, Hayes JD, Henderson CJ, Wolf CR. Identification of retinoic acid as an inhibitor of transcription factor Nrf2 through activation of retinoic acid receptor alpha. Proc Natl Acad Sci U S A. 2007 Dec 4; 104(49):19589-94. Epub 2007 Nov 28. PubMed PMID: 18048326; PubMed Central PMCID: PMC2148333.
Wang XJ, Hayes JD, Wolf CR. Generation of a stable antioxidant response element-driven reporter gene cell line and its use to show redox-dependent activation of nrf2 by cancer chemotherapeutic agents. Cancer Res. 2006 Nov 15;66 (22):10983-94. PubMed PMID: 17108137.

Hyperlink to Supplier’s Cell Line Web Page: 16071902