Description: MDA-MB-157 was established from the pleural effusion of a 44 year old woman with metastatic breast cancer. Desmosomes, microvilli and tonofilaments observed at boundaries between cells. Ethnicity: Black.

Also Known As:

Species: Human

Tissue: Breast

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: L15 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subculturing Procedure: This cell line is relatively slow growing and should be left 5-7 days between splits (media change every 2-3 days). When resuscitating from frozen seed into non-vented flasks at 4-5 x10,000 cells/cm². Split sub-confluent cultures (80-90 %) 1:3 to 1:6, i.e. seeding at 1-3 x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 37°C. CO2 is not required.

Release Conditions: Yes – commercial organisations are required to complete the ‘Cell Line Release Authorisation for Research Use in Commercial Organisations’ release conditions form.

Depositor: The University of Texas M. D. Anderson Cancer Center Office of Technology Commercialization 7515 S. Main, Suite 490, Unit 0510 Houston, Texas 77030

Originator: Yes

References: Young RK, Cailleau RM, Mackay B, Reeves WJ 1974 Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma. In Vitro. 9(4):239-45. PMID: 4471183.

Hyperlink to ECACC Cell Line Data Sheet: 92020422