MDCK
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Description
Description: From kidney of normal female adult Cocker Spaniel in 1958 by SH Madin and NB Darby (Madin Darby Canine Kidney). Supports growth of wide range of animal viruses: VSV (Indiana strain)”, infectious Canine Hepatitis, Vaccinia, Coxsackie B5, Adeno and reo viruses, SVEV.
Also Known As:
Species: Dog
Tissue: Kidney
Growth Properties: Adherent
Morphology: Epithelial
Growth Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3×10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.
Release Conditions: No
Depositor: Dr D Tyrrell, MRC Common Cold Unit, Salisbury
Originator: Yes
References: Proc Soc Exp Biol Med 1958;98:574; Proc Soc Exp Biol Med 1966;122:931
Hyperlink to ECACC Cell Line Data Sheet: 84121903
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