Description: MDCK I – Madin-Darby canine kidney- is a subclone derived from the heterogeneous parent line MDCK (Madin-Darby canine kidney, ECACC catalogue no. 85011435). MDCK-I cells, also known as "low passage MDCK" are reported to display a high electrical resistance (over 1000 Ohm/cm²) and form a monolayer with characteristics of tight epithelia of the renal collecting duct. Grown on filters, the cells show a cuboidal polarised morphology and express microvillae-like projections on the apical surface. Cultures on glass possess junctions but no polarised or columnar morphology. MDCK-1 cells display an adrenalin, vasopressin and prostoglandin E sensitive adenyl cyclase. Please note, these cells were mycoplasma erradicated prior to deposit at ECACC. Epithelial phenotype may be unstable. Incomplete harvesting at confluence may select for smooth-muscle phenotype this is important if cells being used for transepithelial resistance studies.

Also Known As:

Species: Dog

Tissue: Kidney

Growth Properties: Adherent

Morphology: Polarised epithelial

Growth Medium: MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000cells/cm² using 0.25% tryspin or trypsin/EDTA; 5% CO2; 37°C

Release Conditions: No

Depositor:

Originator: No

References: Cell 1984;38:65; Biochem Biophys Acta 1981;673:26,S. Fuller and K. Simons,EMBL, Heidelberg (Fuller et al., 1984), Fuller, Bonsdorff and Simons, Cell 38:65-77,1984, Hansson, Simon and van Meer, EMBO J 5:483-489,1986,”

Hyperlink to ECACC Cell Line Data Sheet: 00062106