MDCK II
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Description
Description: MDCK II – Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line – MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm². This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells. Mycoplasma eradicated using Boehringer method prior to deposit at ECACC.
Also Known As:
Species: Dog
Tissue: Kidney
Growth Properties: Adherent
Morphology: Polarised epithelial
Growth Medium: MEM + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.
Subculturing Procedure: Split sub-confluent cultures (70-80%) every 2-3 days; seeding at 1-3 x 10,000cells/cm² using 0.25% tryspin or trypsin/EDTA; 5% CO2 37°C,
Release Conditions: No
Depositor:
Originator: No
References: Hansson, G.C.,Simons, K and Van Meer G (1986) EMBO 5: 483-489, Louvard D (1980) PNAS 77; 4132-4136
Hyperlink to ECACC Cell Line Data Sheet: 00062107
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