MDST8
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Description
Description: The cell line MDST8, also known as T8, has been established from human colorectal tumour explants. It has been applied in gene therapy studies using adenoviral transduction.
Also Known As:
Species: Human
Tissue: Colon
Growth Properties: Adherent
Morphology: Epithelial-like
Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 seeding at 1-3×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
Release Conditions: No
Depositor: Drs S Todryk, R M Diaz and R G Vile, Division of Oncology, St George’s Hospital Medical School, London, UK
Originator: Yes
References: Gene Therapy 1988;5:869
Hyperlink to ECACC Cell Line Data Sheet: 99011801
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