Description: Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Also Known As:

Species: Human

Tissue: Pleural cavity

Growth Properties: Adherent

Morphology: Spindle shaped cells

Growth Medium: Hams F10 + 15% FCS + 2mM Glutamine

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4×10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 24hrs

Release Conditions: No

Depositor: Dr Marjan A. Versnel, Department of Immunology, Erasmus MC, Dr. Molewaterplein 50, 3015 GE Rotterdam, The Netherlands.

Originator: Yes

References: Versnel et al., 1989 Establishment of human malignant mesothelioma cell lines Int. J. Cancer 44: 256-260 PMID:2474517

Hyperlink to ECACC Cell Line Data Sheet: 09100101