Description: The drug-resistant cell line NCI-H69/LX20 has been derived by exposure of the parent line, H69 (ECACC Number 91091802) to doxorubicin, also known as adriamycin. These multi drug resistant (MDR) cells have been found to be recognised by various anti-P-glycoprotein antibodies.

Also Known As:

Species: Human

Tissue: Lung

Growth Properties: Aggregates in suspension

Morphology:

Growth Medium: RPMI 1640 + 2mM Glutamine + 2µg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS). ECACC has found that the following supplement will assist good growth of cells: sodium selenite 10nM, Insulin 250ng/ml, Transferrin 10ug/ml and sodium pyruvate 1mM.

Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C. It is recommended to culture the cells without drug upon resuscitation until the first passage. Resuscitate into 9 ml of medium. Immediately after resuscitation perform a centrifugation step to remove the cryoprotectant (DMSO) i.e. pellet the cells by centrifugation at 150 x g for 5 minutes and resuspend the cell pellet in fresh medium using the appropriate volume to achieve the correct seeding density (3 -5 x 100,000 viable cells/ml).

Release Conditions: No

Depositor: Dr P Twentyman, UKCCCR, Lincolns Inn Fields, London

Originator: Yes

References: Br J Cancer 1992;65:239

Hyperlink to ECACC Cell Line Data Sheet: 96042332