Description: Mouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion.

Also Known As:

Species: Rat/mouse hybrid

Tissue: neural

Growth Properties: Adherent

Morphology: Neuronal

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3×10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Release Conditions: No

Depositor: Dr J N Wood, Neuroimmunology Dept, The Sandoz Institute, London

Originator: Yes

References: Proc Royal Soc (B) 1990;241:187

Hyperlink to ECACC Cell Line Data Sheet: 92090903