OX-23
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Description
Description: The hybridoma line OX-23 was raised by immunizing BALB/c mice with Factor H and fusion of spleen cells with NS0 mouse myeloma cells. The antibody was shown to detect human complement control protein, Factor H. OX-23, also known as MRC OX-23, reduced the increase in cofactor activity observed in the presence of OX-24 (ECACC catalogue no. 00010403). It was reported that OX-23 and OX-24 react with different epitopes on the Factor H molecule.
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Species: Mouse
Growth Medium: RPMI 1640 + 2mM glutamine + 10% FBS
Subculturing Procedure: Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37°C.
Release Conditions: Yes – commercial organisations are required to complete the ‘Cell Line Release Authorisation for Research Use in Commercial Organisations’ release conditions form
Depositor: Dr. M. Puklavec, Cellular Immunology, Sir William Dunn School of Pathology, Oxford
Originator: Yes
References: Bioscience Reports 1932; 3:1119
Hyperlink to ECACC Cell Line Data Sheet: 00010402
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