Description: P4E6 is an Immortalised human prostate cell line derived from a biopsy of a well-differentiated early stage prostate cancer (Gleason grade (4)) P4E6 was immortalised using human papillomavirus type E6 gene from PLXSN16E6 retroviral stock (Denise Galloway, Fred Hutchinson Cancer Centre Seattle). The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Also Known As:

Species: Human

Tissue: Prostate

Growth Properties:

Morphology: Epithelial

Growth Medium: Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

Release Conditions: Any recipient wanting to use the cell line in the field of vaccines for the treatment or prevention of prostate cancer should first obtain permission from GemVax & KAEL Co., Ltd. Contact : Dr. Eujin Hyun, Head of R&D Department (eujinhyun@kaelgemvax.com)

Depositor: Professor. Norman J Maitland Director YCR Cancer Research Unit Department of Biology (Area 13) University of York Heslington York YO10 5DD

Originator: Yes

References: Maitland NJ, Macintosh CA, Hall J, Sharrard M, Quinn G and Lang S. (2001) In vitro models to study cellular differentiation and function in human prostate cancers. Radiation Research 155, 133-142. PMID: 11121225

Hyperlink to ECACC Cell Line Data Sheet: 10112301