Description: The cell line PEA-10 is heterozygous for a disruption of the gene for low density lipoprotein receptor-related protein (LRP). PEA-10 was derived from fibroblasts from day 15 C57BL/6×129 F1 hybrid mouse embryos. Together with the cell lines PEA-13 (ECACC catalogue no. 97040311) and MEF-1 (ECACC catalogue no. 98061101) an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LRP deficiency. This is of importance since LRP deficient mouse embryos die early during gestation.

Also Known As:

Species: Mouse

Tissue: Unknown

Growth Properties: Adherent

Morphology: Fibroblast

Growth Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculturing Procedure: Split confleunt cultures 1:6 to 1:10 i.e. seeding 2-4 x 10000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C

Release Conditions: No

Depositor:

Originator: No

References: J Cell Sci. 1994; 107:719

Hyperlink to ECACC Cell Line Data Sheet: 97040310